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Measure the antioxidant efficacy of your ingredients, your products
with an ORAC test
The ORAC test allows to determine the antioxidant power of an ingredient, a compound, a formula, based on their capacity to trap free radicals.
Standardized test
Quantitative measurement
Description of the test
The ORAC method aims to evaluate the ability of a sample to limit the oxidation of a fluorescent reagent such as fluorescein which is a probe sensitive to oxidation. The oxidation of fluorescein is accompanied by a decrease in fluorescence measured over time at an excitation wavelength of 485nm and an emission wavelength of 520nm. This change in fluorescence is directly related to the number of free radicals. Indeed, the presence of antioxidants leads to an inhibition of free radicals that damage the fluorescent compound. The standard antioxidant used is TROLOX, a water-soluble vitamin E analog. Fluorescence decay kinetics are assessed every minute for one hour.
Elements
to provide
- Sample to analyze :
– For liquids : 1-5 ml
– For solids : 5-10 mg - Following information for each sample :
– Chemical nature: all chemical natures except radioactive elements.
– Form : solid (granular, powdery, etc …) liquid.
– Solubility : soluble or insoluble in the experimental conditions (culture medium).
– Concentration
– Shipping conditions: depending on the type of molecule and the packaging recommended to maintain its stability.
Results
The higher the ORAC index, the more antioxidant properties the test sample possesses in a quantitative way. This index or relative value of ORAC is defined as a ratio between the difference between the areas under the curves of the test sample and the blank, and the difference between the areas under the curves of the Trolox and the blank. The results are therefore expressed as molarity of Trolox per molarity or mass of sample tested.
