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The FRAP (Ferric Reducing Ability of Plasma) test is used to determine the reducing ability of compounds. It measures the capacity of an antioxidant to reduce ferric iron into ferrous cations. At WAINVAM-E, we offer to perform the FRAP test for you to quantify the power of your antioxidants.
The FRAP assay is a colorimetric electron transfer method for measuring the ability of an antioxidant to reduce the colorless ferric-tripyridyltriazine complex (Fe3+-TPTZ) to the blue ferrous tripyridyltriazine cation ([Fe(II)(TPTZ)2]2+), which absorbs in the UV spectrum at 593 nm. Point measurement in triplicate.
The absorbance value obtained by spectrophotometry is proportional to the antioxidant capacity of the molecule to be tested.